Determination of Platinum in Blood Serum of Chemotherapy Patients by Electrothemal Atomic Absorption Spectrometry

Abstract

The aim of this work was to optimize and validate a simple and rapid method for the direct determination of total platinum in blood serum of chemotherapy patients. The method is based on the quantification of platinum by electrothermal atomic absorption spectrometry (ET-AAS) with the Zeeman background correction after appropriate dilution of samples with 0.2% (v/v) Triton X-100 using the optimized temperature program (pyrolysis and atomization temperatures 1400 °C and 2550 °C, respectively). The validated range of quantification was 0.11–2.15 mmol l^–1, RSD was better than 9 %. The accuracy was checked by comparing the results with those obtained by inductively coupled plasma mass spectrometry (ICP-MS). The optimized ET-AAS method is now successfully used for investigation of some pharmacokinetic parameters in a clinical study comparing intravenous and intraperitoneal infusion of carboplatin (combined with paclitaxel).